Cargando…

Use of dual-transfection for programmed death cell protein 1 disruption mediated by CRISPR-Cas9 in human peripheral blood mononuclear cells

OBJECTIVE(S): Checkpoint blocking is considered a revolutionary method in cancer treatment. This method eliminates cancer cells by maintaining the sensitivity of immune cells. Today, cell therapy through checkpoint blocking is known as the most efficient method of cancer treatment. The programmed ce...

Descripción completa

Detalles Bibliográficos
Autores principales:	Alambeladi, SeyedAli, Hosseiny, SeyedEbrahim, Jafarinia, Mojtaba, Dianatpour, Mehdi
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Mashhad University of Medical Sciences 2021
Materias:	Original Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7894638/ https://www.ncbi.nlm.nih.gov/pubmed/33643569 http://dx.doi.org/10.22038/ijbms.2020.48566.11146

Ejemplares similares

Targeting the MALAT1 gene with the CRISPR/Cas9 technique in prostate cancer
por: Ahmadi-Balootaki, Soraya, et al.
Publicado: (2022)

CRISPR-Cas9 delivery to hard-to-transfect cells via membrane deformation
por: Han, Xin, et al.
Publicado: (2015)

CRISPR/Cas9-mediated knockout of MLL5 enhances apoptotic effect of cisplatin in HeLa cells in vitro
por: Pirouzfar, Mohammad, et al.
Publicado: (2020)

Dystrophin gene editing by CRISPR/Cas9 system in human skeletal muscle cell line (HSkMC)
por: Dara, Mahintaj, et al.
Publicado: (2021)

Using CRISPR/Cas9 System to Knock out Exon 48 in DMD Gene
por: Dara, Mahintaj, et al.
Publicado: (2021)

Optimized RNP transfection for highly efficient CRISPR/Cas9-mediated gene knockout in primary T cells
por: Seki, Akiko, et al.
Publicado: (2018)

CRISPR/Cas9-Mediated TERT Disruption in Cancer Cells
por: Wen, Luan, et al.
Publicado: (2020)

Method for Dual Viral Vector Mediated CRISPR-Cas9 Gene Disruption in Primary Human Endothelial Cells
por: Gong, Haixia, et al.
Publicado: (2017)

Improvement of CRISPR/Cas9 system by transfecting Cas9-expressing Plasmodium berghei with linear donor template
por: Shinzawa, Naoaki, et al.
Publicado: (2020)

Establishment, Culture, and Characterization of Guinea Pig Fetal Fibroblast Cell
por: Mehrabani, Davood, et al.
Publicado: (2014)

A ribonucleoprotein transfection strategy for CRISPR/Cas9‐mediated gene editing and single cell cloning in rainbow trout cells
por: Zoppo, Marina, et al.
Publicado: (2021)

CRISPR/Cas9 ribonucleoprotein (RNP) complex enables higher viability of transfected cells in genome editing of acute myeloid cells
por: Cheng, Qinquan, et al.
Publicado: (2022)

Successful delivery of large-size CRISPR/Cas9 vectors in hard-to-transfect human cells using small plasmids
por: Søndergaard, Jonas Nørskov, et al.
Publicado: (2020)

A review of emerging physical transfection methods for CRISPR/Cas9-mediated gene editing
por: Fajrial, Apresio K., et al.
Publicado: (2020)

Disruptive Technology: CRISPR/Cas-Based Tools and Approaches
por: Patsali, Petros, et al.
Publicado: (2019)

Genetic modification of cystic fibrosis with ΔF508 mutation of CFTR gene using the CRISPR system in peripheral blood mononuclear cells
por: Khatibi, Sepideh, et al.
Publicado: (2021)

A model of metformin mitochondrial metabolism in metachromatic leukodystrophy: first description of human Schwann cells transfected with CRISPR-Cas9
por: Sanchez-Álvarez, Nayibe Tatiana, et al.
Publicado: (2022)

Multiplexed pancreatic genome engineering and cancer induction by transfection-based CRISPR/Cas9 delivery in mice
por: Maresch, Roman, et al.
Publicado: (2016)

Multiplexed CRISPR/Cas9 gene knockout with simple crRNA:tracrRNA co-transfection
por: Khan, Fehad J., et al.
Publicado: (2019)

In Vitro CRISPR/Cas9 Transfection and Gene-Editing Mediated by Multivalent Cationic Liposome–DNA Complexes
por: Sousa, Diana A., et al.
Publicado: (2022)

CRISPR-mediated Transfection of Brugia malayi
por: Liu, Canhui, et al.
Publicado: (2020)

Spermatogenesis after Transplantation of Adipose Tissue-Derived Stem Cells in Azoospermic Guinea Pigs: A Histological and Histomorphometric Study
por: Hajihoseini, Mehrdokht, et al.
Publicado: (2018)

Overexpression of Mitochondrial Genes (Mitochondrial Transcription Factor A and Cytochrome c Oxidase Subunit 1) in Mouse Metaphase II Oocytes following Vitrification via Cryotop
por: Dehghani, Naeimeh, et al.
Publicado: (2019)

BIRC5 Gene Disruption via CRISPR/Cas9n Platform Suppress Acute Myelocytic Leukemia Progression
por: Narimani, Manizheh, et al.
Publicado: (2019)

Protocol for IPO11 deletion and re-expression in H460 lung cancer cells using CRISPR-Cas9 and plasmid transfection
por: Zhang, Yarui, et al.
Publicado: (2023)

Cholesterol-rich lipid-mediated nanoparticles boost of transfection efficiency, utilized for gene editing by CRISPR-Cas9
por: Hosseini, Elaheh Sadat, et al.
Publicado: (2019)

Establishment of targeted mutagenesis in soybean protoplasts using CRISPR/Cas9 RNP delivery via electro−transfection
por: Subburaj, Saminathan, et al.
Publicado: (2023)

Rapid Assessment of CRISPR Transfection Efficiency and Enrichment of CRISPR Induced Mutations Using a Dual-Fluorescent Stable Reporter System
por: Shalaby, Karim E., et al.
Publicado: (2022)

Optimization of Human Peripheral Blood Mononuclear Cells (PBMCs) Cryopreservation
por: Nazarpour, Robab, et al.
Publicado: (2012)

Intrauterine xenotransplantation of human Wharton jelly-derived mesenchymal stem cells into the liver of rabbit fetuses: A preliminary study for in vivo expression of the human liver genes
por: Rezaeian, Leila, et al.
Publicado: (2018)

CaSilico: A versatile CRISPR package for in silico CRISPR RNA designing for Cas12, Cas13, and Cas14
por: Asadbeigi, Adnan, et al.
Publicado: (2022)

Highly Efficient Gene Disruption of Murine and Human Hematopoietic Progenitor Cells by CRISPR/Cas9
por: Brunetti, Lorenzo, et al.
Publicado: (2018)

HEK293T Cells with TFAM Disruption by CRISPR-Cas9 as a Model for Mitochondrial Regulation
por: de Oliveira, Vanessa Cristina, et al.
Publicado: (2021)

Targeted gene disruption in Xenopus laevis using CRISPR/Cas9
por: Wang, Fengqin, et al.
Publicado: (2015)

CRISPR-Cas9 Mediated Exonic Disruption for HIV-1 Elimination
por: Herskovitz, Jonathan, et al.
Publicado: (2021)

Recyclable CRISPR/Cas9 mediated gene disruption and deletions in Histoplasma
por: Joehnk, Bastian, et al.
Publicado: (2023)

Craspase: A novel CRISPR/Cas dual gene editor
por: Huo, George, et al.
Publicado: (2023)

CRISPR-Cas9 mediated efficient PD-1 disruption on human primary T cells for adoptive therapy
por: Su, Shu, et al.
Publicado: (2015)

CRISPR/Cas9-mediated ASXL1 mutations in U937 cells disrupt myeloid differentiation
por: Wu, Zhi-Jie, et al.
Publicado: (2018)

Harnessing the CRISPR/Cas9 system to disrupt latent HIV-1 provirus
por: Ebina, Hirotaka, et al.
Publicado: (2013)

Cannot write session to /tmp/vufind_sessions/sess_tri1la9hrm0l91clcjos5dt9ng