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Direct evaluation of self-quenching behavior of fluorophores at high concentrations using an evanescent field
The quantum yield of a fluorophore is reduced when two or more identical fluorophores are in close proximity to each other. The study of protein folding or particle aggregation is can be done based on this above-mentioned phenomenon—called self-quenching. However, it is challenging to characterize t...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7895399/ https://www.ncbi.nlm.nih.gov/pubmed/33606817 http://dx.doi.org/10.1371/journal.pone.0247326 |