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Direct evaluation of self-quenching behavior of fluorophores at high concentrations using an evanescent field

The quantum yield of a fluorophore is reduced when two or more identical fluorophores are in close proximity to each other. The study of protein folding or particle aggregation is can be done based on this above-mentioned phenomenon—called self-quenching. However, it is challenging to characterize t...

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Detalles Bibliográficos
Autores principales: Bae, Wooli, Yoon, Tae-Young, Jeong, Cherlhyun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7895399/
https://www.ncbi.nlm.nih.gov/pubmed/33606817
http://dx.doi.org/10.1371/journal.pone.0247326