Cas12a-assisted precise targeted cloning using in vivo Cre-lox recombination

Direct cloning represents the most efficient strategy to access the vast number of uncharacterized natural product biosynthetic gene clusters (BGCs) for the discovery of novel bioactive compounds. However, due to their large size, repetitive nature, or high GC-content, large-scale cloning of these B...

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Detalles Bibliográficos
Autores principales: Enghiad, Behnam, Huang, Chunshuai, Guo, Fang, Jiang, Guangde, Wang, Bin, Tabatabaei, S. Kasra, Martin, Teresa A., Zhao, Huimin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7896053/
https://www.ncbi.nlm.nih.gov/pubmed/33608525
http://dx.doi.org/10.1038/s41467-021-21275-4

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7896053/
https://www.ncbi.nlm.nih.gov/pubmed/33608525
http://dx.doi.org/10.1038/s41467-021-21275-4

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