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Efficient production of a functional G protein-coupled receptor in E. coli for structural studies

G protein-coupled receptors (GPCRs) are transmembrane signal transducers which regulate many key physiological process. Since their discovery, their analysis has been limited by difficulties in obtaining sufficient amounts of the receptors in high-quality, functional form from heterologous expressio...

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Detalles Bibliográficos
Autores principales: Abiko, Layara Akemi, Rogowski, Marco, Gautier, Antoine, Schertler, Gebhard, Grzesiek, Stephan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7897205/
https://www.ncbi.nlm.nih.gov/pubmed/33501610
http://dx.doi.org/10.1007/s10858-020-00354-6