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Efficient production of a functional G protein-coupled receptor in E. coli for structural studies
G protein-coupled receptors (GPCRs) are transmembrane signal transducers which regulate many key physiological process. Since their discovery, their analysis has been limited by difficulties in obtaining sufficient amounts of the receptors in high-quality, functional form from heterologous expressio...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Netherlands
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7897205/ https://www.ncbi.nlm.nih.gov/pubmed/33501610 http://dx.doi.org/10.1007/s10858-020-00354-6 |