Guide-target mismatch effects on dCas9–sgRNA binding activity in living bacterial cells

As an effective programmable DNA targeting tool, CRISPR–Cas9 system has been adopted in varieties of biotechnological applications. However, the off-target effects, derived from the tolerance towards guide-target mismatches, are regarded as the major problems in engineering CRISPR systems. To unders...

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Detalles Bibliográficos
Autores principales: Feng, Huibao, Guo, Jiahui, Wang, Tianmin, Zhang, Chong, Xing, Xin-hui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Chemical Biology and Nucleic Acid Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7897480/
https://www.ncbi.nlm.nih.gov/pubmed/33503261
http://dx.doi.org/10.1093/nar/gkaa1295

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7897480/
https://www.ncbi.nlm.nih.gov/pubmed/33503261
http://dx.doi.org/10.1093/nar/gkaa1295

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