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Guide-target mismatch effects on dCas9–sgRNA binding activity in living bacterial cells
As an effective programmable DNA targeting tool, CRISPR–Cas9 system has been adopted in varieties of biotechnological applications. However, the off-target effects, derived from the tolerance towards guide-target mismatches, are regarded as the major problems in engineering CRISPR systems. To unders...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7897480/ https://www.ncbi.nlm.nih.gov/pubmed/33503261 http://dx.doi.org/10.1093/nar/gkaa1295 |