Parallel monitoring of RNA abundance, localization and compactness with correlative single molecule FISH on LR White embedded samples

Single mRNA molecules are frequently detected by single molecule fluorescence in situ hybridization (smFISH) using branched DNA technology. While providing strong and background-reduced signals, the method is inefficient in detecting mRNAs within dense structures, in monitoring mRNA compactness and...

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Detalles Bibliográficos
Autores principales: Kramer, Susanne, Meyer-Natus, Elisabeth, Stigloher, Christian, Thoma, Hanna, Schnaufer, Achim, Engstler, Markus
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7897490/
https://www.ncbi.nlm.nih.gov/pubmed/33275141
http://dx.doi.org/10.1093/nar/gkaa1142

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7897490/
https://www.ncbi.nlm.nih.gov/pubmed/33275141
http://dx.doi.org/10.1093/nar/gkaa1142

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