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Parallel monitoring of RNA abundance, localization and compactness with correlative single molecule FISH on LR White embedded samples
Single mRNA molecules are frequently detected by single molecule fluorescence in situ hybridization (smFISH) using branched DNA technology. While providing strong and background-reduced signals, the method is inefficient in detecting mRNAs within dense structures, in monitoring mRNA compactness and...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2020
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7897490/ https://www.ncbi.nlm.nih.gov/pubmed/33275141 http://dx.doi.org/10.1093/nar/gkaa1142 |