The ubiquitin ligase RNF8 regulates Rho GTPases and promotes cytoskeletal changes and motility in triple‐negative breast cancer cells

The ubiquitin ligase RNF8 is known to induce epithelial‐to‐mesenchymal (EMT) transition and metastasis in triple‐negative breast cancer (TNBC). Besides EMT, Rho GTPases have been shown as key regulators in metastasis. In this study, we investigated the role of RNF8 in regulating Rho GTPases and cell...

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Autores principales: Pereira De Carvalho, Bruno, Chern, Yi‐Jye, He, Jiabei, Chan, Chia‐Hsin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Research Letters
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7898409/
https://www.ncbi.nlm.nih.gov/pubmed/33205415
http://dx.doi.org/10.1002/1873-3468.13999
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author Pereira De Carvalho, Bruno
Chern, Yi‐Jye
He, Jiabei
Chan, Chia‐Hsin
author_facet Pereira De Carvalho, Bruno
Chern, Yi‐Jye
He, Jiabei
Chan, Chia‐Hsin
author_sort Pereira De Carvalho, Bruno
collection PubMed
description The ubiquitin ligase RNF8 is known to induce epithelial‐to‐mesenchymal (EMT) transition and metastasis in triple‐negative breast cancer (TNBC). Besides EMT, Rho GTPases have been shown as key regulators in metastasis. In this study, we investigated the role of RNF8 in regulating Rho GTPases and cell motility. We find that RNF8 knockdown in TNBC cells attenuates the protein and mRNA levels of Ras homolog family member A (RHOA) and cell division cycle 42 (CDC42). We show that the formation of filopodia, focal adhesions, and the association of focal adhesions to stress fibers is impaired upon RNF8 knockdown. Cell migration is significantly inhibited by RNF8 knockdown. Our study suggests a potential novel role for RNF8 in mediating cell migration in TNBC through regulation of the Rho GTPases RHOA and CDC42.
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spelling pubmed-78984092021-03-03 The ubiquitin ligase RNF8 regulates Rho GTPases and promotes cytoskeletal changes and motility in triple‐negative breast cancer cells Pereira De Carvalho, Bruno Chern, Yi‐Jye He, Jiabei Chan, Chia‐Hsin FEBS Lett Research Letters The ubiquitin ligase RNF8 is known to induce epithelial‐to‐mesenchymal (EMT) transition and metastasis in triple‐negative breast cancer (TNBC). Besides EMT, Rho GTPases have been shown as key regulators in metastasis. In this study, we investigated the role of RNF8 in regulating Rho GTPases and cell motility. We find that RNF8 knockdown in TNBC cells attenuates the protein and mRNA levels of Ras homolog family member A (RHOA) and cell division cycle 42 (CDC42). We show that the formation of filopodia, focal adhesions, and the association of focal adhesions to stress fibers is impaired upon RNF8 knockdown. Cell migration is significantly inhibited by RNF8 knockdown. Our study suggests a potential novel role for RNF8 in mediating cell migration in TNBC through regulation of the Rho GTPases RHOA and CDC42. John Wiley and Sons Inc. 2020-12-05 2021-01 /pmc/articles/PMC7898409/ /pubmed/33205415 http://dx.doi.org/10.1002/1873-3468.13999 Text en © 2020 The Authors. FEBS Letters published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Research Letters
Pereira De Carvalho, Bruno
Chern, Yi‐Jye
He, Jiabei
Chan, Chia‐Hsin
The ubiquitin ligase RNF8 regulates Rho GTPases and promotes cytoskeletal changes and motility in triple‐negative breast cancer cells
title The ubiquitin ligase RNF8 regulates Rho GTPases and promotes cytoskeletal changes and motility in triple‐negative breast cancer cells
title_full The ubiquitin ligase RNF8 regulates Rho GTPases and promotes cytoskeletal changes and motility in triple‐negative breast cancer cells
title_fullStr The ubiquitin ligase RNF8 regulates Rho GTPases and promotes cytoskeletal changes and motility in triple‐negative breast cancer cells
title_full_unstemmed The ubiquitin ligase RNF8 regulates Rho GTPases and promotes cytoskeletal changes and motility in triple‐negative breast cancer cells
title_short The ubiquitin ligase RNF8 regulates Rho GTPases and promotes cytoskeletal changes and motility in triple‐negative breast cancer cells
title_sort ubiquitin ligase rnf8 regulates rho gtpases and promotes cytoskeletal changes and motility in triple‐negative breast cancer cells
topic Research Letters
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7898409/
https://www.ncbi.nlm.nih.gov/pubmed/33205415
http://dx.doi.org/10.1002/1873-3468.13999
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