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A high-throughput fluorescence lifetime-based assay to detect binding of myosin-binding protein C to F-actin
Binding properties of actin-binding proteins are typically evaluated by cosedimentation assays. However, this method is time-consuming, involves multiple steps, and has a limited throughput. These shortcomings preclude its use in screening for drugs that modulate actin-binding proteins relevant to h...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Rockefeller University Press
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7898471/ https://www.ncbi.nlm.nih.gov/pubmed/33600558 http://dx.doi.org/10.1085/jgp.202012707 |