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In Situ Labeling and Distance Measurements of Membrane Proteins in E. coli Using Finland and OX063 Trityl Labels

In situ investigation of membrane proteins is a challenging task. Previously we demonstrated that nitroxide labels combined with pulsed ESR spectroscopy is a promising tool for this purpose. However, the nitroxide labels suffer from poor stability, high background labeling, and low sensitivity. Here...

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Detalles Bibliográficos
Autores principales: Ketter, Sophie, Gopinath, Aathira, Rogozhnikova, Olga, Trukhin, Dmitrii, Tormyshev, Victor M., Bagryanskaya, Elena G., Joseph, Benesh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7898545/
https://www.ncbi.nlm.nih.gov/pubmed/33197077
http://dx.doi.org/10.1002/chem.202004606