Cryo-EM grid optimization for membrane proteins

Cryo-EM grid preparation is an important bottleneck in protein structure determination, especially for membrane proteins, typically requiring screening of a large number of conditions. We systematically investigated the effects of buffer components, blotting conditions and grid types on the outcome...

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Detalles Bibliográficos
Autores principales: Kampjut, Domen, Steiner, Julia, Sazanov, Leonid A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7900225/
https://www.ncbi.nlm.nih.gov/pubmed/33665558
http://dx.doi.org/10.1016/j.isci.2021.102139

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7900225/
https://www.ncbi.nlm.nih.gov/pubmed/33665558
http://dx.doi.org/10.1016/j.isci.2021.102139

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