Selective Release of Recombinant Periplasmic Protein From E. coli Using Continuous Pulsed Electric Field Treatment

To date, high-pressure homogenization is the standard method for cell disintegration before the extraction of cytosolic and periplasmic protein from E. coli. Its main drawback, however, is low selectivity and a resulting high load of host cell impurities. Pulsed electric field (PEF) treatment may be...

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Detalles Bibliográficos
Autores principales: Schottroff, Felix, Kastenhofer, Jens, Spadiut, Oliver, Jaeger, Henry, Wurm, David J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Bioengineering and Biotechnology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7900513/
https://www.ncbi.nlm.nih.gov/pubmed/33634078
http://dx.doi.org/10.3389/fbioe.2020.586833

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7900513/
https://www.ncbi.nlm.nih.gov/pubmed/33634078
http://dx.doi.org/10.3389/fbioe.2020.586833

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