Cargando…
Persistence of RNA transcription during DNA replication delays duplication of transcription start sites until G2/M
As transcription and replication use DNA as substrate, conflicts between transcription and replication can occur, leading to genome instability with direct consequences for human health. To determine how the two processes are coordinated throughout S phase, we characterize both processes together at...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cell Press
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7900609/ https://www.ncbi.nlm.nih.gov/pubmed/33596418 http://dx.doi.org/10.1016/j.celrep.2021.108759 |
_version_ | 1783654244131274752 |
---|---|
author | Wang, Jianming Rojas, Patricia Mao, Jingwen Mustè Sadurnì, Martina Garnier, Olivia Xiao, Songshu Higgs, Martin R. Garcia, Paloma Saponaro, Marco |
author_facet | Wang, Jianming Rojas, Patricia Mao, Jingwen Mustè Sadurnì, Martina Garnier, Olivia Xiao, Songshu Higgs, Martin R. Garcia, Paloma Saponaro, Marco |
author_sort | Wang, Jianming |
collection | PubMed |
description | As transcription and replication use DNA as substrate, conflicts between transcription and replication can occur, leading to genome instability with direct consequences for human health. To determine how the two processes are coordinated throughout S phase, we characterize both processes together at high resolution. We find that transcription occurs during DNA replication, with transcription start sites (TSSs) not fully replicated along with surrounding regions and remaining under-replicated until late in the cell cycle. TSSs undergo completion of DNA replication specifically when cells enter mitosis, when RNA polymerase II is removed. Intriguingly, G2/M DNA synthesis occurs at high frequency in unperturbed cell culture, but it is not associated with increased DNA damage and is fundamentally separated from mitotic DNA synthesis. TSSs duplicated in G2/M are characterized by a series of specific features, including high levels of antisense transcription, making them difficult to duplicate during S phase. |
format | Online Article Text |
id | pubmed-7900609 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Cell Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-79006092021-03-03 Persistence of RNA transcription during DNA replication delays duplication of transcription start sites until G2/M Wang, Jianming Rojas, Patricia Mao, Jingwen Mustè Sadurnì, Martina Garnier, Olivia Xiao, Songshu Higgs, Martin R. Garcia, Paloma Saponaro, Marco Cell Rep Article As transcription and replication use DNA as substrate, conflicts between transcription and replication can occur, leading to genome instability with direct consequences for human health. To determine how the two processes are coordinated throughout S phase, we characterize both processes together at high resolution. We find that transcription occurs during DNA replication, with transcription start sites (TSSs) not fully replicated along with surrounding regions and remaining under-replicated until late in the cell cycle. TSSs undergo completion of DNA replication specifically when cells enter mitosis, when RNA polymerase II is removed. Intriguingly, G2/M DNA synthesis occurs at high frequency in unperturbed cell culture, but it is not associated with increased DNA damage and is fundamentally separated from mitotic DNA synthesis. TSSs duplicated in G2/M are characterized by a series of specific features, including high levels of antisense transcription, making them difficult to duplicate during S phase. Cell Press 2021-02-16 /pmc/articles/PMC7900609/ /pubmed/33596418 http://dx.doi.org/10.1016/j.celrep.2021.108759 Text en © 2021 The Author(s) http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Wang, Jianming Rojas, Patricia Mao, Jingwen Mustè Sadurnì, Martina Garnier, Olivia Xiao, Songshu Higgs, Martin R. Garcia, Paloma Saponaro, Marco Persistence of RNA transcription during DNA replication delays duplication of transcription start sites until G2/M |
title | Persistence of RNA transcription during DNA replication delays duplication of transcription start sites until G2/M |
title_full | Persistence of RNA transcription during DNA replication delays duplication of transcription start sites until G2/M |
title_fullStr | Persistence of RNA transcription during DNA replication delays duplication of transcription start sites until G2/M |
title_full_unstemmed | Persistence of RNA transcription during DNA replication delays duplication of transcription start sites until G2/M |
title_short | Persistence of RNA transcription during DNA replication delays duplication of transcription start sites until G2/M |
title_sort | persistence of rna transcription during dna replication delays duplication of transcription start sites until g2/m |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7900609/ https://www.ncbi.nlm.nih.gov/pubmed/33596418 http://dx.doi.org/10.1016/j.celrep.2021.108759 |
work_keys_str_mv | AT wangjianming persistenceofrnatranscriptionduringdnareplicationdelaysduplicationoftranscriptionstartsitesuntilg2m AT rojaspatricia persistenceofrnatranscriptionduringdnareplicationdelaysduplicationoftranscriptionstartsitesuntilg2m AT maojingwen persistenceofrnatranscriptionduringdnareplicationdelaysduplicationoftranscriptionstartsitesuntilg2m AT mustesadurnimartina persistenceofrnatranscriptionduringdnareplicationdelaysduplicationoftranscriptionstartsitesuntilg2m AT garnierolivia persistenceofrnatranscriptionduringdnareplicationdelaysduplicationoftranscriptionstartsitesuntilg2m AT xiaosongshu persistenceofrnatranscriptionduringdnareplicationdelaysduplicationoftranscriptionstartsitesuntilg2m AT higgsmartinr persistenceofrnatranscriptionduringdnareplicationdelaysduplicationoftranscriptionstartsitesuntilg2m AT garciapaloma persistenceofrnatranscriptionduringdnareplicationdelaysduplicationoftranscriptionstartsitesuntilg2m AT saponaromarco persistenceofrnatranscriptionduringdnareplicationdelaysduplicationoftranscriptionstartsitesuntilg2m |