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Cell-free Directed Evolution of a Protease in Microdroplets at Ultrahigh Throughput
[Image: see text] Compartmentalization of single genes in water-in-oil emulsion droplets is a powerful approach to create millions of reactors for enzyme library selections. When these droplets are formed at ultrahigh throughput in microfluidic devices, their perfect monodispersity allows quantitati...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical
Society
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7901014/ https://www.ncbi.nlm.nih.gov/pubmed/33502841 http://dx.doi.org/10.1021/acssynbio.0c00538 |