Cargando…
Cell-free Directed Evolution of a Protease in Microdroplets at Ultrahigh Throughput
[Image: see text] Compartmentalization of single genes in water-in-oil emulsion droplets is a powerful approach to create millions of reactors for enzyme library selections. When these droplets are formed at ultrahigh throughput in microfluidic devices, their perfect monodispersity allows quantitati...
Autores principales: | Holstein, Josephin M., Gylstorff, Christian, Hollfelder, Florian |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical
Society
2021
|
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7901014/ https://www.ncbi.nlm.nih.gov/pubmed/33502841 http://dx.doi.org/10.1021/acssynbio.0c00538 |
Ejemplares similares
-
Ultrahigh-Throughput
Directed Evolution of a Metal-Free
α/β-Hydrolase with a Cys-His-Asp Triad into an Efficient
Phosphotriesterase
por: Schnettler, J. David, et al.
Publicado: (2022) -
A Titratable Cell Lysis-on-Demand System for Droplet-Compartmentalized
Ultrahigh-Throughput Screening in Functional Metagenomics and Directed
Evolution
por: Alex Wong, Che Fai, et al.
Publicado: (2021) -
Ultrahigh-Throughput
Enzyme Engineering and Discovery
in In Vitro Compartments
por: Gantz, Maximilian, et al.
Publicado: (2023) -
Ultrahigh‐Throughput Detection of Enzymatic Alcohol Dehydrogenase Activity in Microfluidic Droplets with a Direct Fluorogenic Assay
por: Klaus, Miriam, et al.
Publicado: (2021) -
“NAD‐display”: Ultrahigh‐Throughput in Vitro Screening of NAD(H) Dehydrogenases Using Bead Display and Flow Cytometry
por: Lindenburg, Laurens, et al.
Publicado: (2021)