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Three-dimensional single molecule localization close to the coverslip: a comparison of methods exploiting supercritical angle fluorescence

The precise spatial localization of single molecules in three dimensions is an important basis for single molecule localization microscopy (SMLM) and tracking. At distances up to a few hundred nanometers from the coverslip, evanescent wave coupling into the glass, also known as supercritical angle f...

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Detalles Bibliográficos
Autores principales: Zelger, Philipp, Bodner, Lisa, Offterdinger, Martin, Velas, Lukas, Schütz, Gerhard J., Jesacher, Alexander
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Optical Society of America 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7901312/
https://www.ncbi.nlm.nih.gov/pubmed/33680543
http://dx.doi.org/10.1364/BOE.413018