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Three-dimensional single molecule localization close to the coverslip: a comparison of methods exploiting supercritical angle fluorescence
The precise spatial localization of single molecules in three dimensions is an important basis for single molecule localization microscopy (SMLM) and tracking. At distances up to a few hundred nanometers from the coverslip, evanescent wave coupling into the glass, also known as supercritical angle f...
Autores principales: | Zelger, Philipp, Bodner, Lisa, Offterdinger, Martin, Velas, Lukas, Schütz, Gerhard J., Jesacher, Alexander |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Optical Society of America
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7901312/ https://www.ncbi.nlm.nih.gov/pubmed/33680543 http://dx.doi.org/10.1364/BOE.413018 |
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