Roadblock-qPCR: a simple and inexpensive strategy for targeted measurements of mRNA stability

The stability of mRNAs is fundamental to determining expression level and dynamics. Nonetheless, current approaches for measuring transcript half-lives (e.g., transcription shutoff) are generally toxic or technically complex. Here we describe an alternative strategy for targeted measurements of endo...

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Detalles Bibliográficos
Autores principales: Watson, Maegan J., Park, Yeonwoo, Thoreen, Carson C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2021
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7901842/
https://www.ncbi.nlm.nih.gov/pubmed/33288682
http://dx.doi.org/10.1261/rna.076885.120

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7901842/
https://www.ncbi.nlm.nih.gov/pubmed/33288682
http://dx.doi.org/10.1261/rna.076885.120

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