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Roadblock-qPCR: a simple and inexpensive strategy for targeted measurements of mRNA stability
The stability of mRNAs is fundamental to determining expression level and dynamics. Nonetheless, current approaches for measuring transcript half-lives (e.g., transcription shutoff) are generally toxic or technically complex. Here we describe an alternative strategy for targeted measurements of endo...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Cold Spring Harbor Laboratory Press
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7901842/ https://www.ncbi.nlm.nih.gov/pubmed/33288682 http://dx.doi.org/10.1261/rna.076885.120 |