Single well, single-common primer pair, dual probe, duplex qPCR assay for the quantification of mRNA splicing variants

Quantifying the ratio of alternatively spliced mRNA variants of genes with known alternative splicing variants is highly relevant for many applications. Herein, we describe the validation of a quantitative PCR design for the simplified quantification of known mRNA splice variants. The assay uses a s...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Janice, Wong, Winifred P, Link, Emma O, Olivares, Shantel, Adelman, Cade T, Henkel, Anne S, El Muayed, Malek
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Innovations
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7903517/
https://www.ncbi.nlm.nih.gov/pubmed/33655078
http://dx.doi.org/10.1093/biomethods/bpab002

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7903517/
https://www.ncbi.nlm.nih.gov/pubmed/33655078
http://dx.doi.org/10.1093/biomethods/bpab002

Ejemplares similares