Cargando…

Single well, single-common primer pair, dual probe, duplex qPCR assay for the quantification of mRNA splicing variants

Quantifying the ratio of alternatively spliced mRNA variants of genes with known alternative splicing variants is highly relevant for many applications. Herein, we describe the validation of a quantitative PCR design for the simplified quantification of known mRNA splice variants. The assay uses a s...

Descripción completa

Detalles Bibliográficos
Autores principales:	Wang, Janice, Wong, Winifred P, Link, Emma O, Olivares, Shantel, Adelman, Cade T, Henkel, Anne S, El Muayed, Malek
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2021
Materias:	Innovations
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7903517/ https://www.ncbi.nlm.nih.gov/pubmed/33655078 http://dx.doi.org/10.1093/biomethods/bpab002

Ejemplares similares

Targeted Single Primer Enrichment Sequencing with Single End Duplex-UMI
por: Peng, Quan, et al.
Publicado: (2019)

Experimental, comparative, duplex mapping study of arterial flow distribution in ischemia and reperfusion by retrograde circulation
por: Busato, Cesar Roberto, et al.
Publicado: (2020)

Determination of Advantages and Limitations of qPCR Duplexing in a Single Fluorescent Channel
por: Zhang, Haoqing, et al.
Publicado: (2021)

Fluorescent-dependent comparative C(t) method for qPCR gene expression analysis in IVF clinical pre-implantation embryonic testing
por: Lal, Arnav, et al.
Publicado: (2021)

Models for prediction of single base primer extension efficiency from position and type of single mismatch in primer-template duplex
por: Ri, Myong-Rim, et al.
Publicado: (2023)

SpeciesPrimer: a bioinformatics pipeline dedicated to the design of qPCR primers for the quantification of bacterial species
por: Dreier, Matthias, et al.
Publicado: (2020)

Laparoscopic transperitoneal heminephrectomy for treatment of the nonfunctioning moiety of duplex kidney in adults: A case series
por: Singh, Manmeet, et al.
Publicado: (2019)

Split-thickness Skin Graft Harvest under Local Anesthetic: A Single Pass Technique
por: Begaj, Ardit, et al.
Publicado: (2021)

SAT064 Establishment And Initial Characterization Of Two Parallel Targeted Human ZnT8 Knock-in Murine Lines For Studying The Type 2 Diabetes Risk Variant At SNP rs13266634 Of The ZnT8 Gene
por: Wang, Janice C, et al.
Publicado: (2023)

A Single Incision Anterior Approach for Transhumeral Amputation Targeted Muscle Reinnervation
por: Daly, Michael C., et al.
Publicado: (2020)

Induction of fibroblast growth factor 21 does not require activation of the hepatic X-box binding protein 1 in mice
por: Olivares, Shantel, et al.
Publicado: (2017)

Endoplasmic reticulum stress induces hepatic plasminogen activator inhibitor 1 in murine nonalcoholic steatohepatitis
por: Olivares, Shantel, et al.
Publicado: (2020)

A reliable method for quantification of splice variants using RT-qPCR
por: Camacho Londoño, Julia, et al.
Publicado: (2016)

qPCR primer design revisited
por: Bustin, Stephen, et al.
Publicado: (2017)

Use of duplex mutation primers for real-time PCR quantification of hepatitis C virus RNA in serum
por: Xia, Qian-Feng, et al.
Publicado: (2011)

Reversed-phase ion-pair liquid chromatography method for purification of duplex DNA with single base pair resolution
por: Wysoczynski, Christina L., et al.
Publicado: (2013)

Modification of the Nuss Procedure: The Single-incision Technique
por: Aizawa, Tetsushi, et al.
Publicado: (2014)

Successful pregnancy outcome after in vitro fertilisation following Pre-implantation Genetic Diagnosis/Polymerase Chain Reaction screening for single gene disorder (sickle cell anaemia) before embryo transfer: The clinical experience of an in vitro fertilisation clinic in Nigeria
por: Okeke, Chizara, et al.
Publicado: (2014)

Design and Validation of qPCR-Specific Primers for Quantification of the Marketed Terfezia claveryi and Terfezia crassiverrucosa in Soil
por: Arenas, Francisco, et al.
Publicado: (2022)

Single access covered endovascular reconstruction of the aortic bifurcation
por: Torrealba, Jose I., et al.
Publicado: (2023)

PrimerBank: a resource of human and mouse PCR primer pairs for gene expression detection and quantification
por: Spandidos, Athanasia, et al.
Publicado: (2010)

BRIE: transcriptome-wide splicing quantification in single cells
por: Huang, Yuanhua, et al.
Publicado: (2017)

No-touch, Single-move Technique to Reverse the Needle While Suturing
por: Algadiem, Emran A.
Publicado: (2016)

Quantification of mRNA in single cells and modelling of RT-qPCR induced noise
por: Bengtsson, Martin, et al.
Publicado: (2008)

High Functional Stability of a Low-cost HBV DNA qPCR Primer Pair and Plasmid Standard
por: Aguiar, Jorge, et al.
Publicado: (2016)

Immediate Single-stage Endoscopic Latissimus Dorsi Breast and Nipple Reconstruction
por: Muñoz i Vidal, Joaquim, et al.
Publicado: (2014)

Single Stage Repair of #30 Facial Cleft with Bone Morphogenic Protein
por: Sheckter, Clifford C., et al.
Publicado: (2018)

A qPCR-duplex assay for sex determination in ancient DNA
por: Poma, Anna, et al.
Publicado: (2022)

Reference genes for quantitative Arabidopsis single molecule RNA fluorescence in situ hybridization
por: Duncan, Susan, et al.
Publicado: (2022)

Endoplasmic Reticulum Stress Activates the Hepatic Activator Protein 1 Complex via Mitogen Activated Protein Kinase-Dependent Signaling Pathways
por: Olivares, Shantel, et al.
Publicado: (2014)

ΩqPCR measures telomere length from single-cells in base pair units
por: Xiong, Fusheng, et al.
Publicado: (2021)

A Single-site Postmarket Retrospective Chart Review of Subdermal Coagulation Procedures with Renuvion
por: Doolabh, Vaishali
Publicado: (2019)

Variance in management of extracorporeal membrane oxygenation-associated fibrin sheaths at a single institution
por: Tedesco, Alexandra, et al.
Publicado: (2023)

Comment on Menke et al. Metals in Urine and Diabetes in U.S. Adults. Diabetes 2016;65:164–171
por: Wong, Winifred P., et al.
Publicado: (2016)

Technique for single-stage reconstruction of obliterative or near-obliterative long urethral strictures in circumcised patients
por: Yadav, Sher Singh, et al.
Publicado: (2018)

A Ready-to-Use Single- and Duplex-TaqMan-qPCR Assay to Detect and Quantify the Biocontrol Agents Trichoderma asperellum and Trichoderma gamsii
por: Gerin, Donato, et al.
Publicado: (2018)

Exploring the Association Between Demographics, SLC30A8 Genotype, and Human Islet Content of Zinc, Cadmium, Copper, Iron, Manganese and Nickel
por: Wong, Winifred P., et al.
Publicado: (2017)

Major vessel sealing in laparoscopic surgery for colorectal cancer: a single-center experience with 759 patients
por: Grieco, Michele, et al.
Publicado: (2018)

A Single-visit Approach Using Fillers and Incobotulinumtoxin A: Full Face Enhancement in Asian Patients
por: Chao, Yates Y. Y.
Publicado: (2018)

Pure single-site robot-assisted pyeloplasty with the da Vinci SP surgical system: Initial experience
por: Heo, Ji Eun, et al.
Publicado: (2019)

Cannot write session to /tmp/vufind_sessions/sess_p7ohh1fq50cs9f5pr60ob5c2c4