Refolding and characterization of two G protein-coupled receptors purified from E. coli inclusion bodies

Aiming at streamlining GPCR production from E. coli inclusion bodies for structural analysis, we present a generic approach to assess and optimize refolding yield through thermostability analysis. Since commonly used hydrophobic dyes cannot be applied as probes for membrane protein unfolding, we ada...

Descripción completa

Detalles Bibliográficos
Autores principales: Heim, Bastian, Handrick, René, Hartmann, Marcus D., Kiefer, Hans
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7904181/
https://www.ncbi.nlm.nih.gov/pubmed/33626080
http://dx.doi.org/10.1371/journal.pone.0247689

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7904181/
https://www.ncbi.nlm.nih.gov/pubmed/33626080
http://dx.doi.org/10.1371/journal.pone.0247689

Ejemplares similares