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Refolding and characterization of two G protein-coupled receptors purified from E. coli inclusion bodies

Aiming at streamlining GPCR production from E. coli inclusion bodies for structural analysis, we present a generic approach to assess and optimize refolding yield through thermostability analysis. Since commonly used hydrophobic dyes cannot be applied as probes for membrane protein unfolding, we ada...

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Detalles Bibliográficos
Autores principales:	Heim, Bastian, Handrick, René, Hartmann, Marcus D., Kiefer, Hans
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2021
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7904181/ https://www.ncbi.nlm.nih.gov/pubmed/33626080 http://dx.doi.org/10.1371/journal.pone.0247689

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