Laboratory Evolution of a Sortase Enzyme that Modifies Amyloid β-protein

Epitope-specific enzymes are powerful tools for site-specific protein modification, but generally require genetic manipulation of the target protein. Here, we describe the laboratory evolution of the bacterial transpeptidase sortase A to recognize the LMVGG sequence in endogenous Aβ protein. Using a...

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Detalles Bibliográficos
Autores principales: Podracky, Christopher J., An, Chihui, DeSousa, Alexandra, Dorr, Brent M., Walsh, Dominic M., Liu, David R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7904614/
https://www.ncbi.nlm.nih.gov/pubmed/33432237
http://dx.doi.org/10.1038/s41589-020-00706-1

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7904614/
https://www.ncbi.nlm.nih.gov/pubmed/33432237
http://dx.doi.org/10.1038/s41589-020-00706-1

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