Extraction-free protocol combining proteinase K and heat inactivation for detection of SARS-CoV-2 by RT-qPCR

Real-time reverse transcription PCR (RT-qPCR) is the gold-standard technique for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) detection in nasopharyngeal swabs specimens. The analysis by RT-qPCR usually requires a previous extraction step to obtain the purified viral RNA. Unfortunate...

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Detalles Bibliográficos
Autores principales: Genoud, Valeria, Stortz, Martin, Waisman, Ariel, Berardino, Bruno G., Verneri, Paula, Dansey, Virginia, Salvatori, Melina, Remes Lenicov, Federico, Levi, Valeria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7909620/
https://www.ncbi.nlm.nih.gov/pubmed/33635936
http://dx.doi.org/10.1371/journal.pone.0247792

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7909620/
https://www.ncbi.nlm.nih.gov/pubmed/33635936
http://dx.doi.org/10.1371/journal.pone.0247792

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