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Cellulase Interacts with Lactic Acid Bacteria to Affect Fermentation Quality, Microbial Community, and Ruminal Degradability in Mixed Silage of Soybean Residue and Corn Stover

SIMPLE SUMMARY: The purpose of this experiment was to investigate the effects of lactic acid bacteria (LAB) and cellulase (CE) on the fermentation quality, bacterial community and ruminal degradability of soybean residue (SR) and corn stover (CS) mixed silage. The fermentation quality, rumen degrada...

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Autores principales: Zhao, Chao, Wang, Lihua, Ma, Guangming, Jiang, Xin, Yang, Jinshan, Lv, Jingyi, Zhang, Yonggen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7912217/
https://www.ncbi.nlm.nih.gov/pubmed/33525728
http://dx.doi.org/10.3390/ani11020334
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author Zhao, Chao
Wang, Lihua
Ma, Guangming
Jiang, Xin
Yang, Jinshan
Lv, Jingyi
Zhang, Yonggen
author_facet Zhao, Chao
Wang, Lihua
Ma, Guangming
Jiang, Xin
Yang, Jinshan
Lv, Jingyi
Zhang, Yonggen
author_sort Zhao, Chao
collection PubMed
description SIMPLE SUMMARY: The purpose of this experiment was to investigate the effects of lactic acid bacteria (LAB) and cellulase (CE) on the fermentation quality, bacterial community and ruminal degradability of soybean residue (SR) and corn stover (CS) mixed silage. The fermentation quality, rumen degradation rate and microbial diversity of mixed storage of SR and CS were effectively improved by adding lactic acid bacteria or cellulase, and the mixed addition of LAB and CE had a better effect, increased the abundance of beneficial lactic acid bacteria and reduced the abundance of harmful microorganisms, including Rahnella. In summary, in long-term mixed silage, the effect of mixed addition of lactic acid bacteria and cellulase was better than that of single addition. ABSTRACT: The objective of this experiment was to investigate the effect of lactic acid bacteria (LAB) and cellulase (CE) on the fermentation quality, rumen degradation rate and bacterial community of mixed silage of soybean residue (SR) and corn stover (CS). The experiment adopted a single-factor experimental design. Four treatment groups were set up: the control group (CON), lactic acid bacteria treatment group (LAB), cellulase treatment group (CE) and lactic acid bacteria + cellulase treatment group (LAB + CE). Among them, the amount of added LAB was 1 × 10(6) CFU/g, and the amount of added CE was 100 U/g. After 56 days of mixed silage, samples were taken and analyzed, and the chemical composition, fermentation quality, rumen degradation rate and microbial diversity were determined. The results showed that the pH of each treatment group was significantly (p < 0.05) lower than that of CON, while the lactic acid and ammoniacal nitrogen contents of each treatment group were significantly higher than that of CON, with the highest contents in the LAB + CE group. The contents of DNFom (Ash-free NDF), ADFom (Ash-free ADF) and DM in the LAB + CE group were significantly lower than those in the CON group, while the content of crude protein (CP) was significantly higher than that in the CON group. The in situ effective degradation rates of DM (ISDMD), DNF (ISNDFD) and CP (ISCPD) were all significantly (p < 0.05) higher in each treatment group than in the control group. The results of principal component analysis showed that the bacterial composition of the LAB, CE and LAB + CE groups was significantly different from that of the CON group (p < 0.05). Bacterial genus level analysis showed that the content of lactic acid bacteria was significantly higher in the LAB + CE group than in the other treatment groups (p < 0.05), while the content of undesirable bacteria was significantly lower than in the other treatment groups. The results showed that the addition of Lactobacillus and/or cellulase in mixed silage of SR and CS could effectively improve the quality of mixed silage fermentation, rumen degradation rate and microbial diversity, with better results when Lactobacillus and cellulase were added together, which provides new ideas for better application of SR and CS in dairy production.
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spelling pubmed-79122172021-02-28 Cellulase Interacts with Lactic Acid Bacteria to Affect Fermentation Quality, Microbial Community, and Ruminal Degradability in Mixed Silage of Soybean Residue and Corn Stover Zhao, Chao Wang, Lihua Ma, Guangming Jiang, Xin Yang, Jinshan Lv, Jingyi Zhang, Yonggen Animals (Basel) Article SIMPLE SUMMARY: The purpose of this experiment was to investigate the effects of lactic acid bacteria (LAB) and cellulase (CE) on the fermentation quality, bacterial community and ruminal degradability of soybean residue (SR) and corn stover (CS) mixed silage. The fermentation quality, rumen degradation rate and microbial diversity of mixed storage of SR and CS were effectively improved by adding lactic acid bacteria or cellulase, and the mixed addition of LAB and CE had a better effect, increased the abundance of beneficial lactic acid bacteria and reduced the abundance of harmful microorganisms, including Rahnella. In summary, in long-term mixed silage, the effect of mixed addition of lactic acid bacteria and cellulase was better than that of single addition. ABSTRACT: The objective of this experiment was to investigate the effect of lactic acid bacteria (LAB) and cellulase (CE) on the fermentation quality, rumen degradation rate and bacterial community of mixed silage of soybean residue (SR) and corn stover (CS). The experiment adopted a single-factor experimental design. Four treatment groups were set up: the control group (CON), lactic acid bacteria treatment group (LAB), cellulase treatment group (CE) and lactic acid bacteria + cellulase treatment group (LAB + CE). Among them, the amount of added LAB was 1 × 10(6) CFU/g, and the amount of added CE was 100 U/g. After 56 days of mixed silage, samples were taken and analyzed, and the chemical composition, fermentation quality, rumen degradation rate and microbial diversity were determined. The results showed that the pH of each treatment group was significantly (p < 0.05) lower than that of CON, while the lactic acid and ammoniacal nitrogen contents of each treatment group were significantly higher than that of CON, with the highest contents in the LAB + CE group. The contents of DNFom (Ash-free NDF), ADFom (Ash-free ADF) and DM in the LAB + CE group were significantly lower than those in the CON group, while the content of crude protein (CP) was significantly higher than that in the CON group. The in situ effective degradation rates of DM (ISDMD), DNF (ISNDFD) and CP (ISCPD) were all significantly (p < 0.05) higher in each treatment group than in the control group. The results of principal component analysis showed that the bacterial composition of the LAB, CE and LAB + CE groups was significantly different from that of the CON group (p < 0.05). Bacterial genus level analysis showed that the content of lactic acid bacteria was significantly higher in the LAB + CE group than in the other treatment groups (p < 0.05), while the content of undesirable bacteria was significantly lower than in the other treatment groups. The results showed that the addition of Lactobacillus and/or cellulase in mixed silage of SR and CS could effectively improve the quality of mixed silage fermentation, rumen degradation rate and microbial diversity, with better results when Lactobacillus and cellulase were added together, which provides new ideas for better application of SR and CS in dairy production. MDPI 2021-01-28 /pmc/articles/PMC7912217/ /pubmed/33525728 http://dx.doi.org/10.3390/ani11020334 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhao, Chao
Wang, Lihua
Ma, Guangming
Jiang, Xin
Yang, Jinshan
Lv, Jingyi
Zhang, Yonggen
Cellulase Interacts with Lactic Acid Bacteria to Affect Fermentation Quality, Microbial Community, and Ruminal Degradability in Mixed Silage of Soybean Residue and Corn Stover
title Cellulase Interacts with Lactic Acid Bacteria to Affect Fermentation Quality, Microbial Community, and Ruminal Degradability in Mixed Silage of Soybean Residue and Corn Stover
title_full Cellulase Interacts with Lactic Acid Bacteria to Affect Fermentation Quality, Microbial Community, and Ruminal Degradability in Mixed Silage of Soybean Residue and Corn Stover
title_fullStr Cellulase Interacts with Lactic Acid Bacteria to Affect Fermentation Quality, Microbial Community, and Ruminal Degradability in Mixed Silage of Soybean Residue and Corn Stover
title_full_unstemmed Cellulase Interacts with Lactic Acid Bacteria to Affect Fermentation Quality, Microbial Community, and Ruminal Degradability in Mixed Silage of Soybean Residue and Corn Stover
title_short Cellulase Interacts with Lactic Acid Bacteria to Affect Fermentation Quality, Microbial Community, and Ruminal Degradability in Mixed Silage of Soybean Residue and Corn Stover
title_sort cellulase interacts with lactic acid bacteria to affect fermentation quality, microbial community, and ruminal degradability in mixed silage of soybean residue and corn stover
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7912217/
https://www.ncbi.nlm.nih.gov/pubmed/33525728
http://dx.doi.org/10.3390/ani11020334
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