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Label-Free Homogeneous microRNA Detection in Cell Culture Medium Based on Graphene Oxide and Specific Fluorescence Quenching

Label-free homogeneous optical detection of low concentration of oligonucleotides using graphene oxide in complex solutions containing proteins remains difficult. We used a colloidal graphene oxide (GO) as a fluorescent probe quencher to detect microRNA-21 spiked-in cell culture medium, overcoming p...

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Detalles Bibliográficos
Autores principales: Nitu, Florentin R., Savu, Lorand, Muraru, Sorin, Stoian, Ioan, Ionită, Mariana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7912907/
https://www.ncbi.nlm.nih.gov/pubmed/33540562
http://dx.doi.org/10.3390/nano11020368