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Marine Fish Primary Hepatocyte Isolation and Culture: New Insights to Enzymatic Dissociation Pancreatin Digestion

Primary cell cultures from wild organisms have been gaining relevance in ecotoxicology as they are considered more sensitive than immortalized cell lines and retain the biochemical pathways found in vivo. In this study, the efficacy of two methods for primary hepatocyte cell isolation was compared u...

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Detalles Bibliográficos
Autores principales: Figueiredo, Neusa, Matos, Beatriz, Diniz, Mário, Branco, Vasco, Martins, Marta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7913162/
https://www.ncbi.nlm.nih.gov/pubmed/33546159
http://dx.doi.org/10.3390/ijerph18041380