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Marine Fish Primary Hepatocyte Isolation and Culture: New Insights to Enzymatic Dissociation Pancreatin Digestion
Primary cell cultures from wild organisms have been gaining relevance in ecotoxicology as they are considered more sensitive than immortalized cell lines and retain the biochemical pathways found in vivo. In this study, the efficacy of two methods for primary hepatocyte cell isolation was compared u...
Autores principales: | Figueiredo, Neusa, Matos, Beatriz, Diniz, Mário, Branco, Vasco, Martins, Marta |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7913162/ https://www.ncbi.nlm.nih.gov/pubmed/33546159 http://dx.doi.org/10.3390/ijerph18041380 |
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