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Optical Monitoring of the Production Quality of Si-Nanoribbon Chips Intended for the Detection of ASD-Associated Oligonucleotides

Gas-phase etching and optical lithography were employed for the fabrication of a silicon nanoribbon chip (Si-NR chip). The quality of the so-fabricated silicon nanoribbons (Si-NRs) was monitored by optical Raman scattering spectroscopy. It was demonstrated that the structures of the Si-NRs were virt...

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Autores principales: Malsagova, Kristina A., Pleshakova, Tatyana O., Popov, Vladimir P., Kupriyanov, Igor N., Galiullin, Rafael A., Kozlov, Andrey F., Shumov, Ivan D., Kaysheva, Anna L., Tikhonenko, Fedor V., Archakov, Alexander I., Ivanov, Yuri D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7913754/
https://www.ncbi.nlm.nih.gov/pubmed/33546438
http://dx.doi.org/10.3390/mi12020147
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author Malsagova, Kristina A.
Pleshakova, Tatyana O.
Popov, Vladimir P.
Kupriyanov, Igor N.
Galiullin, Rafael A.
Kozlov, Andrey F.
Shumov, Ivan D.
Kaysheva, Anna L.
Tikhonenko, Fedor V.
Archakov, Alexander I.
Ivanov, Yuri D.
author_facet Malsagova, Kristina A.
Pleshakova, Tatyana O.
Popov, Vladimir P.
Kupriyanov, Igor N.
Galiullin, Rafael A.
Kozlov, Andrey F.
Shumov, Ivan D.
Kaysheva, Anna L.
Tikhonenko, Fedor V.
Archakov, Alexander I.
Ivanov, Yuri D.
author_sort Malsagova, Kristina A.
collection PubMed
description Gas-phase etching and optical lithography were employed for the fabrication of a silicon nanoribbon chip (Si-NR chip). The quality of the so-fabricated silicon nanoribbons (Si-NRs) was monitored by optical Raman scattering spectroscopy. It was demonstrated that the structures of the Si-NRs were virtually defect-free, meaning they could be used for highly sensitive detection of biological macromolecules. The Si-NR chips were then used for the highly sensitive nanoelectronics detection of DNA oligonucleotides (oDNAs), which represent synthetic analogs of 106a-5p microRNA (miR-106a-5p), associated with the development of autism spectrum disorders in children. The specificity of the analysis was attained by the sensitization of the Si-NR chip sur-face by covalent immobilization of oDNA probes, whose nucleotide sequence was complementary to the known sequence of miR-106a-5p. The use of the Si-NR chip was demonstrated to al-low for the rapid label-free real-time detection of oDNA at ultra-low (~10(−17) M) concentrations.
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spelling pubmed-79137542021-02-28 Optical Monitoring of the Production Quality of Si-Nanoribbon Chips Intended for the Detection of ASD-Associated Oligonucleotides Malsagova, Kristina A. Pleshakova, Tatyana O. Popov, Vladimir P. Kupriyanov, Igor N. Galiullin, Rafael A. Kozlov, Andrey F. Shumov, Ivan D. Kaysheva, Anna L. Tikhonenko, Fedor V. Archakov, Alexander I. Ivanov, Yuri D. Micromachines (Basel) Article Gas-phase etching and optical lithography were employed for the fabrication of a silicon nanoribbon chip (Si-NR chip). The quality of the so-fabricated silicon nanoribbons (Si-NRs) was monitored by optical Raman scattering spectroscopy. It was demonstrated that the structures of the Si-NRs were virtually defect-free, meaning they could be used for highly sensitive detection of biological macromolecules. The Si-NR chips were then used for the highly sensitive nanoelectronics detection of DNA oligonucleotides (oDNAs), which represent synthetic analogs of 106a-5p microRNA (miR-106a-5p), associated with the development of autism spectrum disorders in children. The specificity of the analysis was attained by the sensitization of the Si-NR chip sur-face by covalent immobilization of oDNA probes, whose nucleotide sequence was complementary to the known sequence of miR-106a-5p. The use of the Si-NR chip was demonstrated to al-low for the rapid label-free real-time detection of oDNA at ultra-low (~10(−17) M) concentrations. MDPI 2021-02-03 /pmc/articles/PMC7913754/ /pubmed/33546438 http://dx.doi.org/10.3390/mi12020147 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Malsagova, Kristina A.
Pleshakova, Tatyana O.
Popov, Vladimir P.
Kupriyanov, Igor N.
Galiullin, Rafael A.
Kozlov, Andrey F.
Shumov, Ivan D.
Kaysheva, Anna L.
Tikhonenko, Fedor V.
Archakov, Alexander I.
Ivanov, Yuri D.
Optical Monitoring of the Production Quality of Si-Nanoribbon Chips Intended for the Detection of ASD-Associated Oligonucleotides
title Optical Monitoring of the Production Quality of Si-Nanoribbon Chips Intended for the Detection of ASD-Associated Oligonucleotides
title_full Optical Monitoring of the Production Quality of Si-Nanoribbon Chips Intended for the Detection of ASD-Associated Oligonucleotides
title_fullStr Optical Monitoring of the Production Quality of Si-Nanoribbon Chips Intended for the Detection of ASD-Associated Oligonucleotides
title_full_unstemmed Optical Monitoring of the Production Quality of Si-Nanoribbon Chips Intended for the Detection of ASD-Associated Oligonucleotides
title_short Optical Monitoring of the Production Quality of Si-Nanoribbon Chips Intended for the Detection of ASD-Associated Oligonucleotides
title_sort optical monitoring of the production quality of si-nanoribbon chips intended for the detection of asd-associated oligonucleotides
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7913754/
https://www.ncbi.nlm.nih.gov/pubmed/33546438
http://dx.doi.org/10.3390/mi12020147
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