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Differing Effects of Standard and Harsh Nucleic Acid Extraction Procedures on Diagnostic Helminth Real-Time PCRs Applied to Human Stool Samples

This study aimed to assess standard and harsher nucleic acid extraction schemes for diagnostic helminth real-time PCR approaches from stool samples. A standard procedure for nucleic acid extraction from stool and a procedure including bead-beating as well as proteinase K digestion were compared with...

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Detalles Bibliográficos
Autores principales: Hoffmann, Tanja, Hahn, Andreas, Verweij, Jaco J., Leboulle, Gérard, Landt, Olfert, Strube, Christina, Kann, Simone, Dekker, Denise, May, Jürgen, Frickmann, Hagen, Loderstädt, Ulrike
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7916106/
https://www.ncbi.nlm.nih.gov/pubmed/33572338
http://dx.doi.org/10.3390/pathogens10020188