Apparent Digestibility of Macronutrients and Fatty Acids from Microalgae (Schizochytrium sp.) Fed to Rainbow Trout (Oncorhynchus mykiss): A Potential Candidate for Fish Oil Substitution

SIMPLE SUMMARY: Aquaculture is the world’s fastest growing sector of the global food system. Aquaculture feed producers are seeking substitutes for fishmeal and fish oil to develop more sustainable feeds. Marine microalgae show promise as potential ingredients in aquafeeds; however, the literature lacks data on the digestibility and availability of macronutrients and individual fatty acids for omega 3-rich microalgal biomass, Schizochytrium spp., in rainbow trout. This is an important gap to fill because, among marine microalgae, this species is the most frequently used in fish feed, particularly as a dietary supplement or mixed with other ingredients; digestibility data would guide more judicious inclusion of this microalga biomass. High digestibility of macronutrients, energy and fatty acids showed that Schizochytrium spp. is a high-quality substitute for fish oil and potential candidate as a supplement of LC-PUFA in trout feed with vegetable oils. Digestibility is influenced by temperature and several trials have shown increased macronutrient digestibility with increasing temperature. There is an urgent need to determine the effect of temperature on lipid and fatty acid digestibility. We found that the digestibility of nutrients in rainbow trout maintained at 8 °C or 15 °C was not significantly different. However, the digestibility of DHA omega 3 was significantly higher at 8 °C than at 15 °C. ABSTRACT: Aquaculture feed formulation has recently turned its focus to reduce the reliance on marine-derived resources and utilise alternative feedstuffs, as an approach to improve the environmental sustainability of the aquaculture sector. The fish oil market is highly volatile, and availability of this commodity is continuously decreasing for use in aquaculture. Currently, a growing number of commercial efforts producing microalgae are providing omega 3-rich oil for sustainable aquaculture feed. This study was focused to determine the nutrient digestibility of a marine microalga, Schizochytrium spp., which is rich in docosahexaenoic acid (DHA) and long-chain polyunsaturated fatty acids (LC-PUFA), as a novel dietary lipid source that could be utilized effectively by rainbow trout (Oncorhynchus mykiss). A whole-cell Schizochytrium spp. biomass was used in the digestibility experiment at two different temperatures, 8 °C and 15 °C. No significant differences were detected between the two temperatures for the apparent digestibility coefficients (ADCs) of the dry matter (94.3 ± 4.9%), total lipids (85.8 ± 0.0%), crude proteins (89.5 ± 1.8%), energy (83.1 ± 1.7%) and fatty acids (85.8 ± 7.5%). The ADCs of the nutrients, energy, DHA and other fatty acids showed that Schizochytrium spp. is a high-quality candidate for fish oil substitution and supplement of LC-PUFA in fish feed with vegetable oils.