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Immortalization of human primary prostate epithelial cells via CRISPR inactivation of the CDKN2A locus and expression of telomerase
BACKGROUND: Immortalization of primary prostate epithelial cells (PrEC) with just hTERT expression is particularly inefficient in the absence of DNA tumor viral proteins or p16(INK4A) knockdown. MATERIALS AND METHODS: Here, we describe the establishment of immortalized normal prostate epithelial cel...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7917161/ https://www.ncbi.nlm.nih.gov/pubmed/32873916 http://dx.doi.org/10.1038/s41391-020-00274-4 |