Immortalization of human primary prostate epithelial cells via CRISPR inactivation of the CDKN2A locus and expression of telomerase

BACKGROUND: Immortalization of primary prostate epithelial cells (PrEC) with just hTERT expression is particularly inefficient in the absence of DNA tumor viral proteins or p16(INK4A) knockdown. MATERIALS AND METHODS: Here, we describe the establishment of immortalized normal prostate epithelial cel...

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Detalles Bibliográficos
Autores principales: Zhao, Ziran, Fowle, Holly, Valentine, Henkel, Liu, Zemin, Tan, Yinfei, Pei, Jianming, Badal, Simone, Testa, Joseph R., Graña, Xavier
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7917161/
https://www.ncbi.nlm.nih.gov/pubmed/32873916
http://dx.doi.org/10.1038/s41391-020-00274-4

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