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Comprehensive Analysis of CD4(+) T Cell Responses to CMV pp65 Antigen Restricted by Single HLA-DR, -DQ, and -DP Allotype Within an Individual

Within an individual, six different HLA class II heterodimers are expressed co-dominantly by two alleles of HLA-DR, -DQ, and -DP loci. However, it remained unclear which HLA allotypes were used in T cell responses to a given antigen. For the measurement of the CD4(+) T cell responses restricted by a...

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Detalles Bibliográficos
Autores principales: Hyun, You-Seok, Jo, Hyeong-A, Lee, Yong-Hun, Kim, Sun-Mi, Baek, In-Cheol, Sohn, Hyun-Jung, Cho, Hyun-Il, Kim, Tai-Gyu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7917246/
https://www.ncbi.nlm.nih.gov/pubmed/33658991
http://dx.doi.org/10.3389/fimmu.2020.602014
Descripción
Sumario:Within an individual, six different HLA class II heterodimers are expressed co-dominantly by two alleles of HLA-DR, -DQ, and -DP loci. However, it remained unclear which HLA allotypes were used in T cell responses to a given antigen. For the measurement of the CD4(+) T cell responses restricted by a single HLA allotype, we established a panel of artificial antigen-presenting cells (aAPCs) expressing each single HLA allele of 20 HLA-DRB1, 16 HLA-DQ, and 13 HLA-DP alleles. CD4(+) T cell responses to cytomegalovirus (CMV) pp65 restricted by single HLA class II allotype defined in 45 healthy donors. The average magnitude of CD4(+) T cell responses by HLA-DR allotypes was higher than HLA-DQ and HLA-DP allotypes. CD4(+) T cell responses by DRA*01:01/DRB1*04:06, DQA1*01:02/DQB1*06:02, DPA1*02:02/DPB1*05:01 were higher among the other alleles in each HLA-DR, -DQ, and -DP locus. Interestingly, the frequencies of HLA-DR alleles and the positivity of specific allotypes showed an inverse correlation. One allotype within individuals is dominantly used in CD4(+) T cell response in 49% of donors, and two allotypes showed that in 7% of donors, and any positive response was detected in 44% of donors. Even if one individual had several dominant alleles, CD4(+) T cell responses tended to be restricted by only one of them. Furthermore, CD8(+) and CD4(+) T cell responses by HLA class I and class II were correlated. Our results demonstrate that the CD4(+) T cell preferentially use a few dominant HLA class II allotypes within individuals, similar to CD8(+) T cell response to CMV pp65.