Discovery and Characterization of an ALFA-Tag-Specific Affinity Resin Optimized for Protein Purification at Low Temperatures in Physiological Buffer

Epitope tags are widely employed as tools to detect, purify and manipulate proteins in various experimental systems. We recently introduced the ALFA-tag together with two ALFA-specific single-domain antibodies (sdAbs), NbALFA and NbALFA(PE), featuring high or intermediate affinity, respectively. Tog...

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Detalles Bibliográficos
Autores principales: Kilisch, Markus, Götzke, Hansjörg, Gere-Becker, Maja, Crauel, Alexander, Opazo, Felipe, Frey, Steffen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7918568/
https://www.ncbi.nlm.nih.gov/pubmed/33673130
http://dx.doi.org/10.3390/biom11020269

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7918568/
https://www.ncbi.nlm.nih.gov/pubmed/33673130
http://dx.doi.org/10.3390/biom11020269

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