2passtools: two-pass alignment using machine-learning-filtered splice junctions increases the accuracy of intron detection in long-read RNA sequencing

Transcription of eukaryotic genomes involves complex alternative processing of RNAs. Sequencing of full-length RNAs using long reads reveals the true complexity of processing. However, the relatively high error rates of long-read sequencing technologies can reduce the accuracy of intron identificati...

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Detalles Bibliográficos
Autores principales: Parker, Matthew T., Knop, Katarzyna, Barton, Geoffrey J., Simpson, Gordon G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Software
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7919322/
https://www.ncbi.nlm.nih.gov/pubmed/33648554
http://dx.doi.org/10.1186/s13059-021-02296-0

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7919322/
https://www.ncbi.nlm.nih.gov/pubmed/33648554
http://dx.doi.org/10.1186/s13059-021-02296-0

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