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Effects of storage time and temperature on highly multiparametric flow analysis of peripheral blood samples; implications for clinical trial samples

We sought to determine the effect of time and temperature of blood sample storage before preparation of human peripheral blood mononuclear cells (PBMCs) by Ficoll-hypaque density gradient centrifugation. Blood samples from healthy donors were stored at room temperature (RT) or refrigerated at 4°C be...

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Detalles Bibliográficos
Autores principales: Jerram, Amelia, Guy, Thomas V., Beutler, Lucinda, Gunasegaran, Bavani, Sluyter, Ronald, Fazekas de St Groth, Barbara, McGuire, Helen M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7921292/
https://www.ncbi.nlm.nih.gov/pubmed/33600563
http://dx.doi.org/10.1042/BSR20203827