An optimized protocol for rapid, sensitive and robust on-bead ChIP-seq from primary cells

Integrative analysis of next-generation sequencing data can help understand disease mechanisms. Specifically, ChIP-seq can illuminate where transcription regulators bind to regulate transcription. A major obstacle to performing this assay on primary cells is the low numbers obtained from tissues. Th...

Descripción completa

Detalles Bibliográficos
Autores principales: Texari, Lorane, Spann, Nathanael J., Troutman, Ty D., Sakai, Mashito, Seidman, Jason S., Heinz, Sven
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7921621/
https://www.ncbi.nlm.nih.gov/pubmed/33718886
http://dx.doi.org/10.1016/j.xpro.2021.100358

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7921621/
https://www.ncbi.nlm.nih.gov/pubmed/33718886
http://dx.doi.org/10.1016/j.xpro.2021.100358

Ejemplares similares