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Ameliorative effects of functional crude-chalaza hydrolysates on the hepatosteatosis development induced by a high-fat diet

Approximately 400 metric tons of egg chalazae, a byproduct in the liquid-egg processing plant, are produced yearly but always regarded as a waste in Taiwan. Our team successfully developed a crude egg chalaza hydrolysate by protease-A digestion (CCH-A). Free branched-chain amino acids, 3-aminoisobut...

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Autores principales: Chen, Jr-Wei, Lin, Yi-Ling, Samuel Wu, Yi-Hsieng, Wang, Sheng-Yao, Chou, Chung-Hsi, Chen, Yi-Chen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7921881/
https://www.ncbi.nlm.nih.gov/pubmed/33647717
http://dx.doi.org/10.1016/j.psj.2021.01.031
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author Chen, Jr-Wei
Lin, Yi-Ling
Samuel Wu, Yi-Hsieng
Wang, Sheng-Yao
Chou, Chung-Hsi
Chen, Yi-Chen
author_facet Chen, Jr-Wei
Lin, Yi-Ling
Samuel Wu, Yi-Hsieng
Wang, Sheng-Yao
Chou, Chung-Hsi
Chen, Yi-Chen
author_sort Chen, Jr-Wei
collection PubMed
description Approximately 400 metric tons of egg chalazae, a byproduct in the liquid-egg processing plant, are produced yearly but always regarded as a waste in Taiwan. Our team successfully developed a crude egg chalaza hydrolysate by protease-A digestion (CCH-A). Free branched-chain amino acids, 3-aminoisobutyric acid, and β-alanine, and anserine were assayed in the CCH-A used in this study. Besides, the in vitro bile-acid binding ability and inhibitory lipase activity of CCH-As were demonstrated. Then, high-fat diet feeding for 10 wk caused hyperlipidemia, insulin resistance, and hepatosteatosis in hamsters (P < 0.05). However, CCH-A co-treatment decreased serum/liver triglyceride levels and lipid accumulation in livers by increasing daily fecal lipid/bile-acid outputs, upregulating fatty-acid β oxidation, and downregulating fatty-acid biosynthesis in livers (P < 0.05). CCH-A co-treatment also amended insulin resistance, augmented hepatic antioxidant capacity, and decreased liver damages and inflammatory responses (P < 0.05). Taken together, our results do not only demonstrate the hepatoprotective effects of CCH-As against a chronic high-fat dietary habit, achieving effects similar to Simvastatin, but also decrease the environmental burden of handling chalazae in the liquid-egg industry.
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spelling pubmed-79218812021-03-12 Ameliorative effects of functional crude-chalaza hydrolysates on the hepatosteatosis development induced by a high-fat diet Chen, Jr-Wei Lin, Yi-Ling Samuel Wu, Yi-Hsieng Wang, Sheng-Yao Chou, Chung-Hsi Chen, Yi-Chen Poult Sci PROCESSING AND PRODUCT Approximately 400 metric tons of egg chalazae, a byproduct in the liquid-egg processing plant, are produced yearly but always regarded as a waste in Taiwan. Our team successfully developed a crude egg chalaza hydrolysate by protease-A digestion (CCH-A). Free branched-chain amino acids, 3-aminoisobutyric acid, and β-alanine, and anserine were assayed in the CCH-A used in this study. Besides, the in vitro bile-acid binding ability and inhibitory lipase activity of CCH-As were demonstrated. Then, high-fat diet feeding for 10 wk caused hyperlipidemia, insulin resistance, and hepatosteatosis in hamsters (P < 0.05). However, CCH-A co-treatment decreased serum/liver triglyceride levels and lipid accumulation in livers by increasing daily fecal lipid/bile-acid outputs, upregulating fatty-acid β oxidation, and downregulating fatty-acid biosynthesis in livers (P < 0.05). CCH-A co-treatment also amended insulin resistance, augmented hepatic antioxidant capacity, and decreased liver damages and inflammatory responses (P < 0.05). Taken together, our results do not only demonstrate the hepatoprotective effects of CCH-As against a chronic high-fat dietary habit, achieving effects similar to Simvastatin, but also decrease the environmental burden of handling chalazae in the liquid-egg industry. Elsevier 2021-01-20 /pmc/articles/PMC7921881/ /pubmed/33647717 http://dx.doi.org/10.1016/j.psj.2021.01.031 Text en © 2021 Published by Elsevier Inc. on behalf of Poultry Science Association Inc. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle PROCESSING AND PRODUCT
Chen, Jr-Wei
Lin, Yi-Ling
Samuel Wu, Yi-Hsieng
Wang, Sheng-Yao
Chou, Chung-Hsi
Chen, Yi-Chen
Ameliorative effects of functional crude-chalaza hydrolysates on the hepatosteatosis development induced by a high-fat diet
title Ameliorative effects of functional crude-chalaza hydrolysates on the hepatosteatosis development induced by a high-fat diet
title_full Ameliorative effects of functional crude-chalaza hydrolysates on the hepatosteatosis development induced by a high-fat diet
title_fullStr Ameliorative effects of functional crude-chalaza hydrolysates on the hepatosteatosis development induced by a high-fat diet
title_full_unstemmed Ameliorative effects of functional crude-chalaza hydrolysates on the hepatosteatosis development induced by a high-fat diet
title_short Ameliorative effects of functional crude-chalaza hydrolysates on the hepatosteatosis development induced by a high-fat diet
title_sort ameliorative effects of functional crude-chalaza hydrolysates on the hepatosteatosis development induced by a high-fat diet
topic PROCESSING AND PRODUCT
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7921881/
https://www.ncbi.nlm.nih.gov/pubmed/33647717
http://dx.doi.org/10.1016/j.psj.2021.01.031
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