Discovery of a Novel Member of the Carlavirus Genus from Soybean (Glycine max L. Merr.)

A novel member of the Carlavirus genus, provisionally named soybean carlavirus 1 (SCV1), was discovered by RNA-seq analysis of randomly collected soybean leaves in Illinois, USA. The SCV1 genome contains six open reading frames that encode a viral replicase, triple gene block proteins, a coat protei...

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Autores principales: Thekke-Veetil, Thanuja, McCoppin, Nancy K., Hobbs, Houston A., Hartman, Glen L., Lambert, Kris N., Lim, Hyoun-Sub, Domier, Leslie. L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7922177/
https://www.ncbi.nlm.nih.gov/pubmed/33670683
http://dx.doi.org/10.3390/pathogens10020223
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author Thekke-Veetil, Thanuja
McCoppin, Nancy K.
Hobbs, Houston A.
Hartman, Glen L.
Lambert, Kris N.
Lim, Hyoun-Sub
Domier, Leslie. L.
author_facet Thekke-Veetil, Thanuja
McCoppin, Nancy K.
Hobbs, Houston A.
Hartman, Glen L.
Lambert, Kris N.
Lim, Hyoun-Sub
Domier, Leslie. L.
author_sort Thekke-Veetil, Thanuja
collection PubMed
description A novel member of the Carlavirus genus, provisionally named soybean carlavirus 1 (SCV1), was discovered by RNA-seq analysis of randomly collected soybean leaves in Illinois, USA. The SCV1 genome contains six open reading frames that encode a viral replicase, triple gene block proteins, a coat protein (CP) and a nucleic acid binding protein. The proteins showed highest amino acid sequence identities with the corresponding proteins of red clover carlavirus A (RCCVA). The predicted amino acid sequence of the SCV1 replicase was only 60.6% identical with the replicase of RCCVA, which is below the demarcation criteria for a new species in the family Betaflexiviridae. The predicted replicase and CP amino acid sequences of four SCV1 isolates grouped phylogenetically with those of members of the Carlavirus genus in the family Betaflexiviridae. The features of the encoded proteins, low nucleotide and amino acid sequence identities of the replicase with the closest member, and the phylogenetic grouping suggest SCV1 is a new member of the Carlavirus genus.
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spelling pubmed-79221772021-03-03 Discovery of a Novel Member of the Carlavirus Genus from Soybean (Glycine max L. Merr.) Thekke-Veetil, Thanuja McCoppin, Nancy K. Hobbs, Houston A. Hartman, Glen L. Lambert, Kris N. Lim, Hyoun-Sub Domier, Leslie. L. Pathogens Communication A novel member of the Carlavirus genus, provisionally named soybean carlavirus 1 (SCV1), was discovered by RNA-seq analysis of randomly collected soybean leaves in Illinois, USA. The SCV1 genome contains six open reading frames that encode a viral replicase, triple gene block proteins, a coat protein (CP) and a nucleic acid binding protein. The proteins showed highest amino acid sequence identities with the corresponding proteins of red clover carlavirus A (RCCVA). The predicted amino acid sequence of the SCV1 replicase was only 60.6% identical with the replicase of RCCVA, which is below the demarcation criteria for a new species in the family Betaflexiviridae. The predicted replicase and CP amino acid sequences of four SCV1 isolates grouped phylogenetically with those of members of the Carlavirus genus in the family Betaflexiviridae. The features of the encoded proteins, low nucleotide and amino acid sequence identities of the replicase with the closest member, and the phylogenetic grouping suggest SCV1 is a new member of the Carlavirus genus. MDPI 2021-02-18 /pmc/articles/PMC7922177/ /pubmed/33670683 http://dx.doi.org/10.3390/pathogens10020223 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Thekke-Veetil, Thanuja
McCoppin, Nancy K.
Hobbs, Houston A.
Hartman, Glen L.
Lambert, Kris N.
Lim, Hyoun-Sub
Domier, Leslie. L.
Discovery of a Novel Member of the Carlavirus Genus from Soybean (Glycine max L. Merr.)
title Discovery of a Novel Member of the Carlavirus Genus from Soybean (Glycine max L. Merr.)
title_full Discovery of a Novel Member of the Carlavirus Genus from Soybean (Glycine max L. Merr.)
title_fullStr Discovery of a Novel Member of the Carlavirus Genus from Soybean (Glycine max L. Merr.)
title_full_unstemmed Discovery of a Novel Member of the Carlavirus Genus from Soybean (Glycine max L. Merr.)
title_short Discovery of a Novel Member of the Carlavirus Genus from Soybean (Glycine max L. Merr.)
title_sort discovery of a novel member of the carlavirus genus from soybean (glycine max l. merr.)
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7922177/
https://www.ncbi.nlm.nih.gov/pubmed/33670683
http://dx.doi.org/10.3390/pathogens10020223
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