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Novel RT-ddPCR assays for simultaneous quantification of multiple noncoding and coding regions of SARS-CoV-2 RNA

A hallmark of coronavirus transcription is the generation of negative-sense RNA intermediates that serve as the templates for the synthesis of positive-sense genomic RNA (gRNA) and an array of subgenomic mRNAs (sgRNAs) encompassing sequences arising from discontinuous transcription. Existing PCR-bas...

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Detalles Bibliográficos
Autores principales: Telwatte, Sushama, Kumar, Nitasha, Vallejo-Gracia, Albert, Kumar, G. Renuka, Lu, Chuanyi M., Ott, Melanie, Wong, Joseph K., Yukl, Steven A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier/North-Holland Biomedical Press 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7923865/
https://www.ncbi.nlm.nih.gov/pubmed/33667568
http://dx.doi.org/10.1016/j.jviromet.2021.114115