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Novel RT-ddPCR assays for simultaneous quantification of multiple noncoding and coding regions of SARS-CoV-2 RNA
A hallmark of coronavirus transcription is the generation of negative-sense RNA intermediates that serve as the templates for the synthesis of positive-sense genomic RNA (gRNA) and an array of subgenomic mRNAs (sgRNAs) encompassing sequences arising from discontinuous transcription. Existing PCR-bas...
Autores principales: | Telwatte, Sushama, Kumar, Nitasha, Vallejo-Gracia, Albert, Kumar, G. Renuka, Lu, Chuanyi M., Ott, Melanie, Wong, Joseph K., Yukl, Steven A. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier/North-Holland Biomedical Press
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7923865/ https://www.ncbi.nlm.nih.gov/pubmed/33667568 http://dx.doi.org/10.1016/j.jviromet.2021.114115 |
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