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RanBP2/Nup358 enhances miRNA activity by sumoylating Argonautes
Mutations in RanBP2 (also known as Nup358), one of the main components of the cytoplasmic filaments of the nuclear pore complex, contribute to the overproduction of acute necrotizing encephalopathy (ANE1)-associated cytokines. Here we report that RanBP2 represses the translation of the interleukin 6...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7924746/ https://www.ncbi.nlm.nih.gov/pubmed/33600493 http://dx.doi.org/10.1371/journal.pgen.1009378 |
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author | Shen, Qingtang Wang, Yifan E. Truong, Mathew Mahadevan, Kohila Wu, Jingze J. Zhang, Hui Li, Jiawei Smith, Harrison W. Smibert, Craig A. Palazzo, Alexander F. |
author_facet | Shen, Qingtang Wang, Yifan E. Truong, Mathew Mahadevan, Kohila Wu, Jingze J. Zhang, Hui Li, Jiawei Smith, Harrison W. Smibert, Craig A. Palazzo, Alexander F. |
author_sort | Shen, Qingtang |
collection | PubMed |
description | Mutations in RanBP2 (also known as Nup358), one of the main components of the cytoplasmic filaments of the nuclear pore complex, contribute to the overproduction of acute necrotizing encephalopathy (ANE1)-associated cytokines. Here we report that RanBP2 represses the translation of the interleukin 6 (IL6) mRNA, which encodes a cytokine that is aberrantly up-regulated in ANE1. Our data indicates that soon after its production, the IL6 messenger ribonucleoprotein (mRNP) recruits Argonautes bound to let-7 microRNA. After this mRNP is exported to the cytosol, RanBP2 sumoylates mRNP-associated Argonautes, thereby stabilizing them and enforcing mRNA silencing. Collectively, these results support a model whereby RanBP2 promotes an mRNP remodelling event that is critical for the miRNA-mediated suppression of clinically relevant mRNAs, such as IL6. |
format | Online Article Text |
id | pubmed-7924746 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-79247462021-03-10 RanBP2/Nup358 enhances miRNA activity by sumoylating Argonautes Shen, Qingtang Wang, Yifan E. Truong, Mathew Mahadevan, Kohila Wu, Jingze J. Zhang, Hui Li, Jiawei Smith, Harrison W. Smibert, Craig A. Palazzo, Alexander F. PLoS Genet Research Article Mutations in RanBP2 (also known as Nup358), one of the main components of the cytoplasmic filaments of the nuclear pore complex, contribute to the overproduction of acute necrotizing encephalopathy (ANE1)-associated cytokines. Here we report that RanBP2 represses the translation of the interleukin 6 (IL6) mRNA, which encodes a cytokine that is aberrantly up-regulated in ANE1. Our data indicates that soon after its production, the IL6 messenger ribonucleoprotein (mRNP) recruits Argonautes bound to let-7 microRNA. After this mRNP is exported to the cytosol, RanBP2 sumoylates mRNP-associated Argonautes, thereby stabilizing them and enforcing mRNA silencing. Collectively, these results support a model whereby RanBP2 promotes an mRNP remodelling event that is critical for the miRNA-mediated suppression of clinically relevant mRNAs, such as IL6. Public Library of Science 2021-02-18 /pmc/articles/PMC7924746/ /pubmed/33600493 http://dx.doi.org/10.1371/journal.pgen.1009378 Text en © 2021 Shen et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Shen, Qingtang Wang, Yifan E. Truong, Mathew Mahadevan, Kohila Wu, Jingze J. Zhang, Hui Li, Jiawei Smith, Harrison W. Smibert, Craig A. Palazzo, Alexander F. RanBP2/Nup358 enhances miRNA activity by sumoylating Argonautes |
title | RanBP2/Nup358 enhances miRNA activity by sumoylating Argonautes |
title_full | RanBP2/Nup358 enhances miRNA activity by sumoylating Argonautes |
title_fullStr | RanBP2/Nup358 enhances miRNA activity by sumoylating Argonautes |
title_full_unstemmed | RanBP2/Nup358 enhances miRNA activity by sumoylating Argonautes |
title_short | RanBP2/Nup358 enhances miRNA activity by sumoylating Argonautes |
title_sort | ranbp2/nup358 enhances mirna activity by sumoylating argonautes |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7924746/ https://www.ncbi.nlm.nih.gov/pubmed/33600493 http://dx.doi.org/10.1371/journal.pgen.1009378 |
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