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Optogenetic control of PRC1 reveals its role in chromosome alignment on the spindle by overlap length-dependent forces
During metaphase, chromosome position at the spindle equator is regulated by the forces exerted by kinetochore microtubules and polar ejection forces. However, the role of forces arising from mechanical coupling of sister kinetochore fibers with bridging fibers in chromosome alignment is unknown. He...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7924949/ https://www.ncbi.nlm.nih.gov/pubmed/33480356 http://dx.doi.org/10.7554/eLife.61170 |
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author | Jagrić, Mihaela Risteski, Patrik Martinčić, Jelena Milas, Ana Tolić, Iva M |
author_facet | Jagrić, Mihaela Risteski, Patrik Martinčić, Jelena Milas, Ana Tolić, Iva M |
author_sort | Jagrić, Mihaela |
collection | PubMed |
description | During metaphase, chromosome position at the spindle equator is regulated by the forces exerted by kinetochore microtubules and polar ejection forces. However, the role of forces arising from mechanical coupling of sister kinetochore fibers with bridging fibers in chromosome alignment is unknown. Here, we develop an optogenetic approach for acute removal of PRC1 to partially disassemble bridging fibers and show that they promote chromosome alignment. Tracking of the plus-end protein EB3 revealed longer antiparallel overlaps of bridging microtubules upon PRC1 removal, which was accompanied by misaligned and lagging kinetochores. Kif4A/kinesin-4 and Kif18A/kinesin-8 were found within the bridging fiber and largely lost upon PRC1 removal, suggesting that these proteins regulate the overlap length of bridging microtubules. We propose that PRC1-mediated crosslinking of bridging microtubules and recruitment of kinesins to the bridging fiber promote chromosome alignment by overlap length-dependent forces transmitted to the associated kinetochore fibers. |
format | Online Article Text |
id | pubmed-7924949 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-79249492021-03-03 Optogenetic control of PRC1 reveals its role in chromosome alignment on the spindle by overlap length-dependent forces Jagrić, Mihaela Risteski, Patrik Martinčić, Jelena Milas, Ana Tolić, Iva M eLife Cell Biology During metaphase, chromosome position at the spindle equator is regulated by the forces exerted by kinetochore microtubules and polar ejection forces. However, the role of forces arising from mechanical coupling of sister kinetochore fibers with bridging fibers in chromosome alignment is unknown. Here, we develop an optogenetic approach for acute removal of PRC1 to partially disassemble bridging fibers and show that they promote chromosome alignment. Tracking of the plus-end protein EB3 revealed longer antiparallel overlaps of bridging microtubules upon PRC1 removal, which was accompanied by misaligned and lagging kinetochores. Kif4A/kinesin-4 and Kif18A/kinesin-8 were found within the bridging fiber and largely lost upon PRC1 removal, suggesting that these proteins regulate the overlap length of bridging microtubules. We propose that PRC1-mediated crosslinking of bridging microtubules and recruitment of kinesins to the bridging fiber promote chromosome alignment by overlap length-dependent forces transmitted to the associated kinetochore fibers. eLife Sciences Publications, Ltd 2021-01-22 /pmc/articles/PMC7924949/ /pubmed/33480356 http://dx.doi.org/10.7554/eLife.61170 Text en © 2021, Jagrić et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Cell Biology Jagrić, Mihaela Risteski, Patrik Martinčić, Jelena Milas, Ana Tolić, Iva M Optogenetic control of PRC1 reveals its role in chromosome alignment on the spindle by overlap length-dependent forces |
title | Optogenetic control of PRC1 reveals its role in chromosome alignment on the spindle by overlap length-dependent forces |
title_full | Optogenetic control of PRC1 reveals its role in chromosome alignment on the spindle by overlap length-dependent forces |
title_fullStr | Optogenetic control of PRC1 reveals its role in chromosome alignment on the spindle by overlap length-dependent forces |
title_full_unstemmed | Optogenetic control of PRC1 reveals its role in chromosome alignment on the spindle by overlap length-dependent forces |
title_short | Optogenetic control of PRC1 reveals its role in chromosome alignment on the spindle by overlap length-dependent forces |
title_sort | optogenetic control of prc1 reveals its role in chromosome alignment on the spindle by overlap length-dependent forces |
topic | Cell Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7924949/ https://www.ncbi.nlm.nih.gov/pubmed/33480356 http://dx.doi.org/10.7554/eLife.61170 |
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