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Rapid Generation of a Recombinant Genotype VIII Newcastle Disease Virus (NDV) Using Full-Length Synthetic cDNA

Rescue of (−)ssRNA viruses involves the sequential assembly and cloning of the full-length cDNA, which is often a challenging and time-consuming process. The objective of this study was to develop a novel method to rapidly clone the full-length cDNA of a very virulent NDV by only one assembly step....

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Detalles Bibliográficos
Autores principales: Murulitharan, Kavitha, Yusoff, Khatijah, Omar, Abdul Rahman, Peeters, Ben P. H., Molouki, Aidin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7929900/
https://www.ncbi.nlm.nih.gov/pubmed/33660046
http://dx.doi.org/10.1007/s00284-021-02421-z