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A suitable RNA preparation methodology for whole transcriptome shotgun sequencing harvested from Plasmodium vivax-infected patients

Plasmodium vivax is a world-threatening human malaria parasite, whose biology remains elusive. The unavailability of in vitro culture, and the difficulties in getting a high number of pure parasites makes RNA isolation in quantity and quality a challenge. Here, a methodological outline for RNA-seq f...

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Detalles Bibliográficos
Autores principales: Bourgard, Catarina, Lopes, Stefanie C. P., Lacerda, Marcus V. G., Albrecht, Letusa, Costa, Fabio T. M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7930272/
https://www.ncbi.nlm.nih.gov/pubmed/33658571
http://dx.doi.org/10.1038/s41598-021-84607-w