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High‐efficiency transformation of archaea by direct PCR products with its application to directed evolution of a thermostable enzyme

Hyperthermophilic archaea with unique biochemical and physiological characteristics are important organisms for fundamental research of life science and have great potential for biotechnological applications. However, low transformation efficiency of foreign DNA molecules impedes developments in gen...

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Detalles Bibliográficos
Autores principales: Song, Yunhong, Zhu, Zhiguang, Zhou, Wei, Zhang, Yi‐Heng P. Job
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7936305/
https://www.ncbi.nlm.nih.gov/pubmed/32602260
http://dx.doi.org/10.1111/1751-7915.13613